General description

Maleimide is an unsaturated imide used in protein conjugation reactions.

Principle

The Fluorometric Maleimide Assay Kit provides a sensitive, one-step fluorometric assay to detect maleimide, which reacts with a proprietary dye, resulting in a fluorometric product (λex = 490/λem = 520 nm) proportional to the amount of maleimide present. The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.

Suitability

The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.